Imaging Chloride Homeostasis in Neurons

Arosio, Daniele (2017) Imaging Chloride Homeostasis in Neurons. PhD thesis, University of Trento.

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Abstract

Intracellular chloride and pH are fundamental regulators of neuronal excitability and they are often co-modulated during excitation-inhibition activity. The study of their homeostasis requires simultaneous measurements in vivo in multiple neurons. Combining random mutagenesis screening, protein engineering and two-photon-imaging this thesis work led to the discovery of new chloride-sensitive GFP mutants and to the establishment of ratiometric imaging procedures for the quantitative combined imaging of intraneuronal pH and chloride. These achievements have been demonstrated in vivo in the mouse cortex, in real-time monitoring the dynamic changes of ions concentrations during epileptic-like discharges, and in glioblastoma primary cells, measuring osmotic swelling responses to various drugs treatment.

Item Type:Doctoral Thesis (PhD)
Doctoral School:Biomolecular Sciences
PhD Cycle:29
Subjects:Area 05 - Scienze biologiche > BIO/11 BIOLOGIA MOLECOLARE
Area 02 - Scienze fisiche > FIS/07 FISICA APPLICATA (A BENI CULTURALI, AMBIENTALI, BIOLOGIA E MEDICINA)
Area 05 - Scienze biologiche > BIO/10 BIOCHIMICA
Area 05 - Scienze biologiche > BIO/13 BIOLOGIA APPLICATA
Repository Staff approval on:07 Mar 2017 09:18

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